Genotyping and primer details


Genotyping and primer details for SALK_047862c - At5g38150
Line ID SALK_047862c
Locus At5g38150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15223483 of line SALK_047862c Chr5:15223483
   
Insertion allele amplicon LBb3 - 43wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 43wm: 5'-GGTTTTGAAAGAAGTGACTGAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15224064
   
wt amplicon (over InsPos) 23xa - 43wm
wt amplicon start Chr5:15222921
wt amplicon end Chr5:15224064
wt amplicon size 1144 bp
Locus specific primer 43wm: 5'-GGTTTTGAAAGAAGTGACTGAAGG-3'
2nd locus specific primer 23xa: 5'-CAAATAAATGTTGAAAGCCAATGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11