Sequences from confirmation amplicon


Confirmation sequences for line SALK_047862c and hit At5g38150 are shown.
Line ID SALK_047862c
Locus At5g38150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15223483
Primers used for PCR 43wm - LBb3, show primer details
   
Sequence Name 42-K118709-SALK_047862C-43wm
GenBank Accession LN485342 [GenBank]
Sequencing primer gene specific primer, 43wm: 5'-GGTTTTGAAAGAAGTGACTGAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K118709-SALK_047862C-43wm
TAGTAAGATCTTCAGGGTCATGACAGTAATGGATGCGTTGAGGAATGAGATTATCCGAGC
CAGGGATGAAACAGCTTGCCTTGGAAAAATCTTGAGGGAAGATGATGTTAAGATTGAGAA
ACTCAACTCAAAGATTCTTATCGAAAAATCGAAGTTGGAAGTGGTATCTATCGCCGAAGA
AAGGATTAGCTCTCTTGCTGAGAATTTCGTTGGTTCGCTTGAGAAGATAAAGAAAAGTAG
AAATGCTGCAAAGAAAGAGGAGTTCCTTTTCAAAGAAGAGAAGACGGTTACAAAGGCGGA
AACACAGAAAACCAAACTTGATATAGATAAAAAGGAAAGCGAACTGAATTCAAAGTTGGA
TGAGCTTGAAAAAGTGAAGCACACTGAGGCTTTAGTGCTTGAGAAGCTTGAATCTTTAGT
AGAAGACATGATGGAGAGTCGCGAAATGGAGTCTGAGCATTGTTCAACAATCACTATCTC
GAGGTTTGAGTACGAGTATTTGAGTAAACATGCTTCACAAGCGGAAGAAACAAATAACAC
ATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAA
TGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACC
GCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAA
TCCTGTTTGATGGTGGTTCCG
   
Sequence Name 17-K118708-SALK_047862C-R210
GenBank Accession LN484764 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118708-SALK_047862C-R210
GTCATTTGTTACACCACAATGTGTTATTTGTTTCTTCCGCTTGTGAAGCATGTTTACTCA
AATACTCGTACTCAAACCTCGAGATAGTGATTGTTGAACAATGCTCAGACTCCATTTCGC
GACTCTCCATCATGTCTTCTACTAAAGATTCAAGCTTCTCAAGCACTAAAGCCTCAGTGT
GCTTCACTTTTTCAAGCTCATCCAACTTTGAATTCAGTTCGCTTTCCTTTTTATCTATAT
CAAGTTTGGTTTTCTGTGTTTCCGCCTTTGTAACCGTCTTCTCTTCTTTGAAAAGGAACT
CCTCTTTCTTTGCAGCATTTCTACTTTTCTTTATCTTCTCAAGCGAACCAACGAAATTCT
CAGCAAGAGAGCTAATCCTTTCTTCGGCGATAGATACCACTTCCAACTTCGATTTTTCGA
TAAGAATCTTTGAGTTGAGTTTCTCAATCTTAACATCATCTTCCCTCAAGATTTTTCCAA
GGCAAGCTGTTTCATCCCTGGCTCGGATAATCTCATTCCTCAACGCATCCATTACTGTCA
TGACCCTGAAGATCTCTACTTTAACAGAAGCAAGTTCCTCCTTCTTCCCTTCAGTCACTT
TTTTCAAAAC

Last Updated on 06.12.2018 16:11