Genotyping and primer details


Genotyping and primer details for SALK_049965c - At4g17660
Line ID SALK_049965c
Locus At4g17660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9832820 of line SALK_049965c Chr4:9832820
   
Insertion allele amplicon LBb3 - 29jr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29jr: 5'-ATTGCTCAGAGGACAGAGAGAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9832046
   
wt amplicon (over InsPos) 08ll - 29jr
wt amplicon start Chr4:9832046
wt amplicon end Chr4:9833100
wt amplicon size 1055 bp
Locus specific primer 29jr: 5'-ATTGCTCAGAGGACAGAGAGAGAC-3'
2nd locus specific primer 08ll: 5'-AACACAAACTTTCTGGTTAATGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11