Genotyping and primer details


Genotyping and primer details for SALK_050213 - At5g56760
Line ID SALK_050213
Locus At5g56760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22962575 of line SALK_050213 Chr5:22962575
   
Insertion allele amplicon LBb3 - 56cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 56cs: 5'-ATTCAAATTCAACAAAGCCAAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22961996
   
wt amplicon (over InsPos) 05dz - 56cs
wt amplicon start Chr5:22961996
wt amplicon end Chr5:22962675
wt amplicon size 680 bp
Locus specific primer 56cs: 5'-ATTCAAATTCAACAAAGCCAAATC-3'
2nd locus specific primer 05dz: 5'-GATTTAAAAATTTCCGCACGATTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11