Genotyping and primer details


Genotyping and primer details for SALK_054181c - At2g31450
Line ID SALK_054181c
Locus At2g31450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13402214 of line SALK_054181c Chr2:13402214
   
Insertion allele amplicon LBb3 - 11en
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 11en: 5'-TTAAGGTAGAAGAATTGGCGAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13401558
   
wt amplicon (over InsPos) 16gc - 11en
wt amplicon start Chr2:13401558
wt amplicon end Chr2:13402534
wt amplicon size 977 bp
Locus specific primer 11en: 5'-TTAAGGTAGAAGAATTGGCGAGAA-3'
2nd locus specific primer 16gc: 5'-CTGTCTTGCTAGGAGCACTTCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11