Sequences from confirmation amplicon


Confirmation sequences for line SALK_054181c and hit At2g31450 are shown.
Line ID SALK_054181c
Locus At2g31450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13402214
Primers used for PCR 11en - LBb3, show primer details
   
Sequence Name 30-K111273-SALK_054181C-11en
GenBank Accession LN485127 [GenBank]
Sequencing primer gene specific primer, 11en: 5'-TTAAGGTAGAAGAATTGGCGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K111273-SALK_054181C-11en
GTATGGCACCATTCTTCCTTTGGAGCCATTGTTGCAAAGCTACTCTTGTTTCCTCTGGAG
ATGTAGTTTTCTAGAAAGTCATAGATTGCAAATGGGTGACAAAGACCAGGCAAACAGAAT
CGGAGACAATCTGCTCGGTATGTCACAATACCATTTCTGATTGAAGCCAATTTAGAGGTA
CCTGTTTGGTTCCTGGACGAGACACCCAACCAAGTCGATTGCAAATGCGATGCACATGTG
TGTCTACACATATGCCTTGGACATCATTCCATGCTATATGCAGAATCTGCCATGTAGAGA
ACAGAAGAGAAATTTGAGAAGCTTTTTGAAAACATTTCATCTAAAACTCACAAGATATAA
GTAATAATATAGTCGAATTTTACCAGATGGGCCATCTTGGGACCAATCCCTGGAAGAGAC
AGCAGGTCGTCCAAAGAGCTTGGAATGTCTCCGTCATATTTCACTAGACAAATTCTAGCT
ATTTTCTTCATATAGGTAGCCTTCCTTGTATAAAATCCAACCTGAAAGTAAAGGCAAACT
GTTAAACCAAGATTTTACTAAGAATAAGGCCTTTTTATGAGAAAATGGCATAAAATTAGC
ACATCAAGGGGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTT
AATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCA
CCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAA
AATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 14-K111272-SALK_054181C-R210
GenBank Accession LN484664 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K111272-SALK_054181C-R210
CATTTGTTACCCCTTGATGTGCTAATTTTATGCCATTTTCTCATAAAAAGGCCTTATTCT
TAGTAAAATCTTGGTTTAACAGTTTGCCTTTACTTTCAGGTTGGATTTTATACAAGGAAG
GCTACCTATATGAAGAAAATAGCTAGAATTTGTCTAGTGAAATATGACGGAGACATTCCA
AGCTCTTTGGACGACCTGCTGTCTCTTCCAGGGATTGGTCCCAAGATGGCCCATCTGGTA
AAATTCGACTATATTATTACTTATATCTTGTGAGTTTTAGATGAAATGTTTTCAAAAAGC
TTCTCAAATTTCTCTTCTGTTCTCTACATGGCAGATTCTGCATATAGCATGGAATGATGT
CCAAGGCATATGTGTAGACACACATGTGCATCGCATTTGCAATCGACTTGGTTGGGTGTC
TCGTCCAGGAACCAAACAGGTACCTCTAAATTGGCTTCAATCAGAAATGGTATTGTGACA
TACCGAGCAGATTGTCTCCGATTCTGTTTGCCTGGTCTTTGTCACCCATTTGCAATCTAT
GACTTTCTAGAAAACTACATCTCCAGAGGAAACAAGAGTAGCTTTGCAACAATGGCTTCC
AAAGGAAGAATGGGTTGCCATTAACCCGCTTTTGGTTCGTCATTCTCGCCAATTCTCTTA
ACCTT

Last Updated on 06.12.2018 16:11