Genotyping and primer details


Genotyping and primer details for SALK_055597c - At2g13820
Line ID SALK_055597c
Locus At2g13820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:5775326 of line SALK_055597c Chr2:5775326
   
Insertion allele amplicon LBb3 - 57us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 57us: 5'-TTGTTGAATCAGCTTTTGAAATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:5775910
   
wt amplicon (over InsPos) 39vl - 57us
wt amplicon start Chr2:5774589
wt amplicon end Chr2:5775910
wt amplicon size 1322 bp
Locus specific primer 57us: 5'-TTGTTGAATCAGCTTTTGAAATTG-3'
2nd locus specific primer 39vl: 5'-TTAACGGAAACTTAACAACATGCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11