Genotyping and primer details


Genotyping and primer details for SALK_057447c - At1g55340
Line ID SALK_057447c
Locus At1g55340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20653509 of line SALK_057447c Chr1:20653509
   
Insertion allele amplicon LBb3 - 37um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 37um: 5'-AGGAACCTGTGAAAGAAACAAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20653018
   
wt amplicon (over InsPos) 32vb - 37um
wt amplicon start Chr1:20653018
wt amplicon end Chr1:20653875
wt amplicon size 858 bp
Locus specific primer 37um: 5'-AGGAACCTGTGAAAGAAACAAAGA-3'
2nd locus specific primer 32vb: 5'-TAATCTGTGTGCAGATTTCGTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11