Sequences from confirmation amplicon


Confirmation sequences for line SALK_057447c and hit At1g55340 are shown.
Line ID SALK_057447c
Locus At1g55340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20653509
Primers used for PCR 37um - LBb3, show primer details
   
Sequence Name 04-K117319-SALK_057447C-37um
GenBank Accession LN484382 [GenBank]
Sequencing primer gene specific primer, 37um: 5'-AGGAACCTGTGAAAGAAACAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117319-SALK_057447C-37um
CTTTGTCAATAAGAGAAGGAAGAAGATTTTTTGGCTATGAAAGGTTGCAAGCTTCCTCAA
AGACCTAAGAAGCGAGCCAAATTGGTTCAGAAGACATTGCTTGTGAGTCTCTTTGAAAAC
TTAACGTTTGTGTTCTTCATAACCATTTTTATCTCATGATTGTTATGTATAATGCAGCTG
GTGAGTCCAGGAGCTTGGTTATCAGATCTGTGCAAAGAGAGATATGAAGTAAGAGAGAAG
AAGACTTCAAAGAAGGTATCTATCAGATTTTTCTCTTTCTAGTTTCTCTGTAGAAGTTAA
TGTTTATGTACTAGCAGAGAGGTGTGATACATTCATATTTTCATGTAGAGACCAAGAGGA
TTGAAGGCAATGGGGAGCATGGAAAGCGATTCGGAGTGAAGAACCGTCGAAGAAAGAACT
TAAATGAGATATTCAAGATATTCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGAC
AACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGT
GAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGG
TCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 20-K117318-SALK_057447C-R210
GenBank Accession LN484864 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117318-SALK_057447C-R210
ACCACAATATATCCTGAATATCTTGAATATCTCATTTAAGTTCTTTCTTCGACGGTTCTT
CACTCCGAATCGCTTTCCATGCTCCCCATTGCCTTCAATCCTCTTGGTCTCTACATGAAA
ATATGAATGTATCACACCTCTCTGCTAGTACATAAACATTAACTTCTACAGAGAAACTAG
AAAGAGAAAAATCTGATAGATACCTTCTTTGAAGTCTTCTTCTCTCTTACTTCATATCTC
TCTTTGCACAGATCTGATAACCAAGCTCCTGGACTCACCAGCTGCATTATACATAACAAT
CATGAGATAAAAATGGTTATGAAGAACACAAACGTTAAGTTTTCAAAGAGACTCACAAGC
AATGTCTTCTGAACCAATTTGGCTCGCTTCTTAGGTCTTTGAGGAAGCTTGCAACCTTTC
ATAGCCAAAAAATCTTCTTCCTTCTCTTTATTTGACAAAGCTATATACAACTTTGGCCAC
ACATGCTTCTTTGTTTCTTTCCAAAGTTCCT

Last Updated on 06.12.2018 16:11