Genotyping and primer details


Genotyping and primer details for SALK_058951c - At1g10700
Line ID SALK_058951c
Locus At1g10700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3554415 of line SALK_058951c Chr1:3554415
   
Insertion allele amplicon R204 - CF42
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CF42: 5'-CGAATTTCCCTATCCACATTCTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3553833
   
Insertion allele amplicon LBb3 - CF43
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CF43: 5'-TGCTTAAAAGCCTAAATGCAAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3554726
   
wt amplicon (over InsPos) CF43 - CF42
wt amplicon start Chr1:3553833
wt amplicon end Chr1:3554726
wt amplicon size 894 bp
Locus specific primer CF42: 5'-CGAATTTCCCTATCCACATTCTAA-3'
2nd locus specific primer CF43: 5'-TGCTTAAAAGCCTAAATGCAAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11