Sequences from confirmation amplicon


Confirmation sequences for line SALK_058951c and hit At1g10700 are shown.
Line ID SALK_058951c
Locus At1g10700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3554415
Primers used for PCR CF42 - R204, show primer details
   
Sequence Name 10-K107118-SALK_058951C-R210
GenBank Accession LN484539 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K107118-SALK_058951C-R210
GCGTCATTTGTTACCCACAATATATCCATGGTTATAGGAGACGATGAATTCGGATTAATT
AGGCTGATCGGATCCCCATTGGATAAGAAATCGAGGCTCCGGTGACCAGAGTGGTTCTCT
AAGCTTCTGACTCCGCACTTGATGCAGCGATTACTGACTGAAGCAGTTTTGAAATTAAGA
AACGATGGAGACGAAGAAGAAGATAACGAGGAAGAAGTTGAGCTAAATTGAGGCAGGGAG
AGTGAAGCAGCGGTGGTTGCATTCGCCGGAGAAATAGCCGCCATTTCCGAAGACGAAGGG
TCAAATTTACGAATTTTCCGGCGGATATCTTCAAATTAAATATACCGAATTTTTTTCGTT
TATAAAACAAATCTGACATGTGGCGGAGACTCCGAAATGACGTAAATGCCCCTATCTGTC
AAATGAGACACGTTATGAAGATGTTGGATAGTTTCGGTTTAATTGGCTAAGTAGCTAACC
ATCGGTTCAAGTTTAATTTGTCCGAATACCAAAAACGAATAGCAGCACTCTGAAACTAAT
TTGTTCAAGACTTGCTCGAAATGAAACGGTTTGTCTCGGTTTAGTTAGAATG
   
Sequence Name 26-K107119-SALK_058951C-CF42
GenBank Accession LN485022 [GenBank]
Sequencing primer gene specific primer, CF42: 5'-CGAATTTCCCTATCCACATTCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K107119-SALK_058951C-CF42
AACAAATTAGTTTTCAGAGTGCTGCTATTCGTTTTTGGTATTCGGACAAATTAAACTTGA
ACCGATGGTTAGCTACTTAGCCAATTAAACCGAAACTATCCAACATCTTCATAACGTGTC
TCATTTGACAGATAGGGGCATTTACGTCATTTCGGAGTCTCCGCCACATGTCAGATTTGT
TTTATAAACGAAAAAAATTCGGTATATTTAATTTGAAGATATCCGCCGGAAAATTCGTAA
ATTTGACCCTTCGTCTTCGGAAATGGCGGCTATTTCTCCGGCGAATGCAACCACCGCTGC
TTCACTCTCCCTGCCTCAATTTAGCTCAACTTCTTCCTCGTTATCTTCTTCTTCGTCTCC
ATCGTTTCTTAATTTCAAAACTGCTTCAGTCAGTAATCGCTGCATCAAGTGCGGAGTCAG
AAGCTTAGAGAACCACTCTGGTCACCGGAGCCTCGATTTCTTATCCAATGGGGATCCGAT
CAGCCTAATTAATCCGAATTCATCGTCTCCTATAACCATGGATATATTGTGGTGTAAACA
AATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTT
TTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAG
AGTTGCAC
Primers used for PCR CF43 - LBb3, show primer details
   
Sequence Name 81-K118526-SALK_058951C-CF43
GenBank Accession LN486238 [GenBank]
Sequencing primer gene specific primer, CF43: 5'-TGCTTAAAAGCCTAAATGCAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K118526-SALK_058951C-CF43
TCTCAGGTTCTCGAATTTGAGCAACAGAATAAGAGCATTGACCAAAAACACATACGAAAC
TCATCTGAAAGTTCAAATTTTTAAGCAAAAGACATCAAATTGATAGAGAGATTGATGGGT
TATTACTTCCAATTGATGCTTCGTAGCTCAATGCAATCAGATTTTGCGACGATTCTCTCG
GCGAGATCTCTAGTCTCGTCGCTGTGGAAGAGACAGACGCGCTTAGAGCTCTTCGAACCG
GATTCCGACGTCGCCGCGGCAATAACAACTTAATAACACATACGCTTAGACAACTTAATA
ACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGC
AACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTG
GTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAAT
   
Sequence Name 71-K118525-SALK_058951C-R210
GenBank Accession LN486042 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K118525-SALK_058951C-R210
CCGCGGCGACNTCGGAATCCGGGTCGAAGAGCTCTAAGCGCGTCTGTCTCTTCCCAGCGA
CGAGACTAGAGATCTCGCCGAGAGAATCGTCGCAAAATCTGATTGCATTGAGCTACGAAG
CATCAATTGGAAGTAATAACCCATCAATCTCTCTATCAATTTGATGTCTTTTGCTTAAAA
ATTTGAACTTTCAGATGAGTTTCGTATGTGTTTTTGGTCAATGCTCTTATTCTGTTGCTC
AAATTCGAGAACCTTGAGATGAGATAGCTCTAAGTCACCGTAAGGAATTTTGCATTTAGG
CTTTTAAGC

Last Updated on 06.12.2018 16:11