Genotyping and primer details


Genotyping and primer details for SALK_060558c - At4g29240
Line ID SALK_060558c
Locus At4g29240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14418327 of line SALK_060558c Chr4:14418327
   
Insertion allele amplicon LBb3 - 36dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 36dr: 5'-TTGCTGCCTTTCTTCTTCTTAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14418851
   
wt amplicon (over InsPos) 08fg - 36dr
wt amplicon start Chr4:14417781
wt amplicon end Chr4:14418851
wt amplicon size 1071 bp
Locus specific primer 36dr: 5'-TTGCTGCCTTTCTTCTTCTTAGAC-3'
2nd locus specific primer 08fg: 5'-GACCACAAACTGTTTTCCATTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11