Genotyping and primer details


Genotyping and primer details for SALK_063974c - At1g20780
Line ID SALK_063974c
Locus At1g20780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7220288 of line SALK_063974c Chr1:7220288
   
Insertion allele amplicon LBb3 - 49cy
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 49cy: 5'-TTATTTAAAGGGTGCGAAGAAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7220954
   
wt amplicon (over InsPos) 08dz - 49cy
wt amplicon start Chr1:7220035
wt amplicon end Chr1:7220954
wt amplicon size 920 bp
Locus specific primer 49cy: 5'-TTATTTAAAGGGTGCGAAGAAAGA-3'
2nd locus specific primer 08dz: 5'-CCATGGCTTTAGAGAATCTTTCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11