Genotyping and primer details


Genotyping and primer details for SALK_064167c - At1g02370
Line ID SALK_064167c
Locus At1g02370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:476374 of line SALK_064167c Chr1:476374
   
Insertion allele amplicon LBb3 - 53dl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 53dl: 5'-TTTCCTAACTTAGTTGGTGGCACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:476698
   
wt amplicon (over InsPos) 18ec - 53dl
wt amplicon start Chr1:475851
wt amplicon end Chr1:476698
wt amplicon size 848 bp
Locus specific primer 53dl: 5'-TTTCCTAACTTAGTTGGTGGCACT-3'
2nd locus specific primer 18ec: 5'-TAGGCGACTTAGATGGGATTAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11