Genotyping and primer details


Genotyping and primer details for SALK_066841c - At4g34950
Line ID SALK_066841c
Locus At4g34950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16643621 of line SALK_066841c Chr4:16643621
   
Insertion allele amplicon LBb3 - 71kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 71kh: 5'-TTCACGGATCTATGTAATGCTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16644197
   
wt amplicon (over InsPos) 09lz - 71kh
wt amplicon start Chr4:16643168
wt amplicon end Chr4:16644197
wt amplicon size 1030 bp
Locus specific primer 71kh: 5'-TTCACGGATCTATGTAATGCTCTG-3'
2nd locus specific primer 09lz: 5'-ACCGGTCTAGTGTTAGACAAGGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11