Sequences from confirmation amplicon


Confirmation sequences for line SALK_066841c and hit At4g34950 are shown.
Line ID SALK_066841c
Locus At4g34950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16643621
Primers used for PCR 71kh - LBb3, show primer details
   
Sequence Name 75-K113914-SALK_066841C-71kh
GenBank Accession LN486131 [GenBank]
Sequencing primer gene specific primer, 71kh: 5'-TTCACGGATCTATGTAATGCTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K113914-SALK_066841C-71kh
CCTTTTGCCGTTTGTCTCACGGCGGTTTTCTTCCCTCCGTGAAATCCCTCCGTCTACTAC
CTTCGCCGAGGATAACGAAGAGTCTAAATACTTTGCTGTGTTTAACATCGTTGCGGTTGT
TGTTGCTGTGTACCTTCAGTCTTACGACATCATCGGAATCAAAACAGGAGCTTTCTCAAT
CGCATTCGCTTCCATACTTCTCATACTCTTAGCCTCTCCTGTCGCTGTACCTTTCCACGC
TTTTATCCGTAGCAAAGTTCATGATGAGCAAGACGTAGAAGGACGAATAGATGAACCTTT
ACTAAGATCAGGATCTGAGATTGAAGTGGAGGAAACAATCGTAGGTGCTGCAGCGGCGGC
GGATAACGAATTGCCACCGTCTCTTAAGCCGTTAAGTAACGAGGAGGAAGAGAATCACGG
AACTATAGTGACGACGGAGAAGAAAAGACCGGTTCTTGGAGAAGAACACACCATAATGGA
AGCTATGTTGACCGTTGACTTTTGGGTGTTGTTCGTGTCGCTTAGACAAATTGACGCTTA
GACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACC
AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAG
CGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
   
Sequence Name 64-K113913-SALK_066841C-R210
GenBank Accession LN485849 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K113913-SALK_066841C-R210
ACACCCAAAAGTTAACGGTTAACATAGCTTCCATTAAGGTGAGTTCTTCTCCAAGAACCG
GTCTTTTCTTCTCCGTCGTCACTATAGTTCCGTGATTCTCTTCCTCCTCGTTACTTAACG
GCTTAAGAGACGGTGGCAATTCGTTATCCGCCGCCGCTGCAGCACCTACGATTGTTTCCT
CCACTTCAATCTCAGATCCTGATCTTAGTAAAGGTTCATCTATTCGTCCTTCTACGTCTT
GCTCATCATGAACTTTGCTACGGATAAAAGCGTGGAAAGGTACAGCGACAGGAGAGGCTA
AGAGTATGAGAAGTATGGAAGCGAATGCGATTGAGAAAGCTCCTGTTTTGATTCCGATGA
TGTCGTAAGACTGAAGGTACACAGCAACAACAACCGCAACGATGTTAAACACAGCAAAGT
ATTTAGACTCTTCGTTATCCTCGGCGAAGGTAGTAGACGGAGGGATTTCACGGAGGAAGA
AAACCGCCGTGAGACAAACGGCAAAAGGCACGACGGAGAGGAGGACAAGAAACGAAGCTG
GGTCAGAGGAAAACAGAGCA

Last Updated on 06.12.2018 16:11