Genotyping and primer details


Genotyping and primer details for SALK_068977c - At1g31070
Line ID SALK_068977c
Locus At1g31070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11085493 of line SALK_068977c Chr1:11085493
   
Insertion allele amplicon LBb3 - 34mh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34mh: 5'-GATATTGAGGTTCGTGATTTCGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11085149
   
wt amplicon (over InsPos) 46rd - 34mh
wt amplicon start Chr1:11085149
wt amplicon end Chr1:11086023
wt amplicon size 875 bp
Locus specific primer 34mh: 5'-GATATTGAGGTTCGTGATTTCGAT-3'
2nd locus specific primer 46rd: 5'-CTTCCTTACAACACACGAAACAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11