Genotyping and primer details


Genotyping and primer details for SALK_069496c - At5g53360
Line ID SALK_069496c
Locus At5g53360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21648924 of line SALK_069496c Chr5:21648924
   
Insertion allele amplicon LBb3 - 04lu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 04lu: 5'-TCAAAAACACCAATTCTCATCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21648145
   
wt amplicon (over InsPos) 19mu - 04lu
wt amplicon start Chr5:21648145
wt amplicon end Chr5:21648992
wt amplicon size 848 bp
Locus specific primer 04lu: 5'-TCAAAAACACCAATTCTCATCAAC-3'
2nd locus specific primer 19mu: 5'-AATCTCAGAAACGCCATGTAAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11