Genotyping and primer details


Genotyping and primer details for SALK_078726c - At4g25835
Line ID SALK_078726c
Locus At4g25835 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13136982 of line SALK_078726c Chr4:13136982
   
Insertion allele amplicon LBb3 - 59ew
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 59ew: 5'-TTCTCTCATCTCCCTCTCTCTGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13136092
   
wt amplicon (over InsPos) 46gc - 59ew
wt amplicon start Chr4:13136092
wt amplicon end Chr4:13137226
wt amplicon size 1135 bp
Locus specific primer 59ew: 5'-TTCTCTCATCTCCCTCTCTCTGAC-3'
2nd locus specific primer 46gc: 5'-AGGATCAAGCTTCTCAATGTGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11