Genotyping and primer details


Genotyping and primer details for SALK_078758 - At3g19740
Line ID SALK_078758
Locus At3g19740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6862977 of line SALK_078758 Chr3:6862977
   
Insertion allele amplicon LBb3 - 30gd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30gd: 5'-TATTACTTTTCTCCTTCCCGGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6862585
   
wt amplicon (over InsPos) 02hy - 30gd
wt amplicon start Chr3:6862585
wt amplicon end Chr3:6863673
wt amplicon size 1089 bp
Locus specific primer 30gd: 5'-TATTACTTTTCTCCTTCCCGGTTT-3'
2nd locus specific primer 02hy: 5'-GGTACGATTGTAAATCCGACAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11