Genotyping and primer details


Genotyping and primer details for SALK_079139 - At4g37690
Line ID SALK_079139
Locus At4g37690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17708609 of line SALK_079139 Chr4:17708609
   
Insertion allele amplicon LBb3 - 62us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 62us: 5'-TAAAGCAAATAAATCAGAGCCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17709153
   
wt amplicon (over InsPos) 36vl - 62us
wt amplicon start Chr4:17708281
wt amplicon end Chr4:17709153
wt amplicon size 873 bp
Locus specific primer 62us: 5'-TAAAGCAAATAAATCAGAGCCGTT-3'
2nd locus specific primer 36vl: 5'-AATCTCAGTCTCATCATCTCCGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11