Genotyping and primer details


Genotyping and primer details for SALK_086345c - At3g49320
Line ID SALK_086345c
Locus At3g49320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18290622 of line SALK_086345c Chr3:18290622
   
Insertion allele amplicon LBb3 - 13lu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13lu: 5'-CATTTGGATTTGATCCTGAGTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18291010
   
wt amplicon (over InsPos) 15mu - 13lu
wt amplicon start Chr3:18290032
wt amplicon end Chr3:18291010
wt amplicon size 979 bp
Locus specific primer 13lu: 5'-CATTTGGATTTGATCCTGAGTTTT-3'
2nd locus specific primer 15mu: 5'-CCTTATAGACAAGCCCAGCACTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11