Genotyping and primer details


Genotyping and primer details for SALK_086402c - At3g03860
Line ID SALK_086402c
Locus At3g03860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:992829 of line SALK_086402c Chr3:992829
   
Insertion allele amplicon LBb3 - 49fm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 49fm: 5'-GTTGTTTCTGCTCCCAAATAAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:992244
   
wt amplicon (over InsPos) 08gs - 49fm
wt amplicon start Chr3:992244
wt amplicon end Chr3:992938
wt amplicon size 695 bp
Locus specific primer 49fm: 5'-GTTGTTTCTGCTCCCAAATAAAAA-3'
2nd locus specific primer 08gs: 5'-TTTGCTATATCATATTTGCGGACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11