Genotyping and primer details


Genotyping and primer details for SALK_087652c - At1g55930
Line ID SALK_087652c
Locus At1g55930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20918790 of line SALK_087652c Chr1:20918790
   
Insertion allele amplicon LBb3 - 33ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33ea: 5'-AGCTAGAGGAGCTAAGGGAAAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20918114
   
wt amplicon (over InsPos) 04fg - 33ea
wt amplicon start Chr1:20918114
wt amplicon end Chr1:20919065
wt amplicon size 952 bp
Locus specific primer 33ea: 5'-AGCTAGAGGAGCTAAGGGAAAAGA-3'
2nd locus specific primer 04fg: 5'-TCTAATAAAACTGGAATTGGACGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11