Sequences from confirmation amplicon


Confirmation sequences for line SALK_087652c and hit At1g55930 are shown.
Line ID SALK_087652c
Locus At1g55930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20918790
Primers used for PCR 33ea - LBb3, show primer details
   
Sequence Name 67-K110715-SALK_087652C-33ea
GenBank Accession LN485924 [GenBank]
Sequencing primer gene specific primer, 33ea: 5'-AGCTAGAGGAGCTAAGGGAAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K110715-SALK_087652C-33ea
GTTGCCTGACTCCGAGATATTGGATCAAGTTGTAGGATAATTGTGGAACTGCAAGAAGCA
GAGCACGTAGCATCTGAGGAGAAGATGAATTCAGATGCCACAGAAGAGAGAGACTTATCA
ATCAAAGGGGCAGCAAAACATGAGCCGAGAGCCACTTACCAACACTGCAAAAATAGAAGA
ATAGTTTTGCAAGTCTTGTAGAATTCAATAAAATGTTACTTTTTGGTTCAATTCTCTTTT
GATTATCACAAAAACTATTGTTTAGAACTAATTTCACAAAATTAGATTCACACTTTCTTC
GTTTCCTTGATCTTTTCAAGTAATTTTTTTTTAGAGCACACATCCATCTGTCTGTGCCCT
GTGGTGTTAGAAAAGGAACTACCAACTGTAAATTATTTGGGTTGTTTCGACAACCGAGTT
TTGGGTACCAAACCAATTAAACCGGTCTGGTATGGCTGAACGGCGCATAAGGGTATTATT
GTCATTTTACTATCTTTCTGTCACCTGCTCGACGGAGGAAAAGACGCCGCCTTTCTTCTT
CTCTGCAAACATTTCACGAGTGACTCCGAACTCAGATAAATCTTAACTCTCAGTTCCATT
GGTGTTTCGACCAACAGCCACAATGTGTTATTATATTGTGGTGTAAACAAATTGACGCTT
AGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCAC
CAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAA
GCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 51-K110714-SALK_087652C-R210
GenBank Accession LN485532 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K110714-SALK_087652C-R210
CGTCATTTGTTTCACCACAATATAATAACACATTGTGGCTGTTGGTCGAAACACCAATGG
AACTGAGAGTTAAGATTTATCTGAGTTCGGAGTCACTCGTGAAATGTTTGCAGAGAAGAA
GAAAGGCGGCGTCTTTTCCTCCGTCGAGCAGGTGACAGAAAGATAGTAAAATGACAATAA
TACCCTTATGCGCCGTTCAGCCATACCAGACCGGTTTAATTGGTTTGGTACCCAAAACTC
GGTTGTCGAAACAACCCAAATAATTTACAGTTGGTAGTTCCTTTTCTAACACCACAGGGC
ACAGACAGATGGATGTGTGCTCTAAAAAAAAATTACTTGAAAAGATCAAGGAAACGAAGA
AAGTGTGAATCTAATTTTGTGAAATTAGTTCTAAACAATAGTTTTTGTGATAATCAAAAG
AGAATTGAACCAAAAAGTAACATTTTATTGAATTCTACAAGACTTGCAAAACTATTCTTC
TATTTTTGCAGTGTTGGTAAGTGGCTCTCGGCTCATGTTTTGCTGCCCCTTTGATTGATA
AGTCTCTCTCTTCTGTGGCATCTGAATTCATCTTCTCCTCAGATGCTACGTGCTCTGCTT
CTTGCAGTTCCACAATTATCCTACAACTTGATCCAATATCTCTGGAGTCAGGCAACACTA
CCCTCTCTGGTGATTCTGTTTTCATCTTTTCCCTTAGCTCCCTCTAG

Last Updated on 06.12.2018 16:11