Genotyping and primer details


Genotyping and primer details for SALK_091618c - At1g04770
Line ID SALK_091618c
Locus At1g04770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1337110 of line SALK_091618c Chr1:1337110
   
Insertion allele amplicon R204 - AW11
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AW11: 5'-GAGATTTTGGATTTGATGTTTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1337584
   
Insertion allele amplicon R212 - AW12
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer AW12: 5'-ACACACAGAGAATCATCAACAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1336549
   
wt amplicon (over InsPos) AW11 - AW12
wt amplicon start Chr1:1336549
wt amplicon end Chr1:1337584
wt amplicon size 1036 bp
Locus specific primer AW12: 5'-ACACACAGAGAATCATCAACAAGC-3'
2nd locus specific primer AW11: 5'-GAGATTTTGGATTTGATGTTTGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11