Sequences from confirmation amplicon


Confirmation sequences for line SALK_091618c and hit At1g04770 are shown.
Line ID SALK_091618c
Locus At1g04770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1337110
Primers used for PCR AW12 - R212, show primer details
   
Sequence Name 77-K106742-SALK_091618C-R214
GenBank Accession LN486166 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K106742-SALK_091618C-R214
CGTGACTCCCTTAATTCTCCGCTCATGATCAGATCGTTGTTTCCCGCCTTCAGTTTAAAC
TATCAGTGTTTGACTACTAATCTCACGACTAGTCTTTAGTTGTGATTAGATTTCTCAAGT
CTCTTGATCAGTGAACTGATGGAATTTGAATGAATGTGGTGTTACAGGGGAACTTGGGAT
GGGCTTATATGCAACTAATGGACTATACAGCAGCTGAAGCTGTGTATCGGAAAGCTCAAT
TGATAGAGCCAGATGCAAACAAGGCTTGCAATTTATGCACATGTCTCATCAAGCAAGGGA
AGCACGATGAGGCGAGGTCGATTCTCTTTCGTGATGTACTAATGGAGAACAAAGAAGGGT
CTGGTGATCCGAGGTTGATGGCTCGGGTTCGAGAACTATTGAGTGAGCTAAAGCCACAGG
AAGAGGAAACAGCTGCTTC
   
Sequence Name 93-K106743-SALK_091618C-AW12
GenBank Accession LN486350 [GenBank]
Sequencing primer gene specific primer, AW12: 5'-ACACACAGAGAATCATCAACAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K106743-SALK_091618C-AW12
CATCGATACCTTTTAAAGATTCCANAGAGATAGAAGCCGCTGCTTCTTCTTCTTGTGGTT
TTAGCGTACTCTATAGTCTCTTGAACCCGAGAAATTAATCTCGGATCACCATACCCTTCT
TTGTTCTCCATTAGTACATCTTGAAAGAGAATCGACTTCGTCTTTTCGTGCTTCCCTTGC
TTGAGGAGACATGTGCATAAATTGTAAGCCTTGTTTGCATCTGGGTCTATCAATTGAGCT
CTCCGATATATAGCTTCTGTCGCTGTATAGTTCATTATTTGTATATAAGAACACTCCTAG
TTTCCCTGTGTACCCCATTTTT
Primers used for PCR AW11 - R204, show primer details
   
Sequence Name 76-K106742-SALK_091618C-R210
GenBank Accession LN486145 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K106742-SALK_091618C-R210
CATTTGTTACACCACGACGGTGACCTGAAACTTCTTGCCATGAGATCTCCCATTCTTTGT
TGGTTTGCCATTGAATGCTTCTCCTTGATATATCATCCAAAGCTTTTGCTTCAATAACTC
AACTTGCTCTTCTATTCTCCCACATTTCTGTTCAACAAAATTCGGAATCCCAAGTTTGTC
AATTTCACTATCTAATAAAACGGTAACCGAGAGAAACAAGAGAATGGTCTCGGTCAGAGT
TTAGTTACCTTATATAGATCGATGAGGACATTGTCTAATGACTCTTGAGCTTGTCTTGAA
CAAAGATCTCTAAAGGATTGAATAGCGTCAATGGCTTCTTCAGCTCTGTTCTGTTGTTTC
ATTAACAAGGCCATGTCTTTAAGAGCACTATCAACTCTATCTCTCGCTTTAATCGCTATC
CAAAACAACTCTATCGCTGCTTCTGCATCTTTCTCCACCAACTGAACCAAACATCAAATC
CAAAATCTC

Last Updated on 06.12.2018 16:11