Genotyping and primer details

Genotyping and primer details for SALK_094943 - At3g02990
Line ID SALK_094943
Locus At3g02990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:675200 of line SALK_094943 Chr3:675200
Insertion allele amplicon LBb3 - 10cn
Locus specific primer 10cn: 5'-TTCAGGAAGCAACTCATTTAAGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:675778
wt amplicon (over InsPos) 19dn - 10cn
wt amplicon start Chr3:674876
wt amplicon end Chr3:675778
wt amplicon size 903 bp
Locus specific primer 10cn: 5'-TTCAGGAAGCAACTCATTTAAGGT-3'
2nd locus specific primer 19dn: 5'-TTTTTAAGAGGCCAAAAGCAAATA-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11