Sequences from confirmation amplicon


Confirmation sequences for line SALK_094943 and hit At3g02990 are shown.
Line ID SALK_094943
Locus At3g02990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:675200
Primers used for PCR 10cn - LBb3, show primer details
   
Sequence Name 64-K108702-SALK_094943C-10cn
GenBank Accession LN485840 [GenBank]
Sequencing primer gene specific primer, 10cn: 5'-TTCAGGAAGCAACTCATTTAAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K108702-SALK_094943C-10cn
GCATCCAAACAGCCATTATTGGATCCAACACTCTCCATTATCTGTCTCGCATCCACAAGA
TCCGAACTTGAGCTCCCAGAAGCTGCTGCTCCTTGCGTTGGAGGTAAGAGATCACCTTGA
GAAAAAGGCAAATTGGTCTCCATGACTTGATTTGTTTCACAAGGCTCGTGGTACTTCATT
GCAGGATAGAAGCCTGCAGGAATGGATGAAACATCTGCCAATGTAACTTCAGGTGATCCA
TTTGAGGAGCTCCCATTGTCTGAAATGTTAGAATTTGGAACATCGCCCAAAAGAAAGCTA
CCATTATTGCTTGAGAGAGATTCATGGCTAGGTGCATTACTCATCTGCTGTATCTGTTGA
AGCATAGTGTTTGTTGCATCGTTCATCGATGACTGATATCTCACAATCTGGCGGCTAAGA
CCATTAACCCCATGACTGCCAGAATTCATCTGGTCCTCAACAGGTAGCCTCCTCTTTTTG
TTGCTTTCAGAAATGTGTTGGTTTGCTTCATTACTCTGCTGAGAAAATTGCTTCATTACA
ATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG
AAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 56-K108701-SALK_094943C-R210
GenBank Accession LN485639 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K108701-SALK_094943C-R210
GTCATTTGTTTCACCACAATATATTGTAATGAAGCAATTTTCTCAGCAGAGTAATGAAGC
AAACCAACACATTTCTGAAAGCAACAAAAAGAGGAGGCTACCTGTTGAGGACCAGATGAA
TTCTGGCAGTCATGGGGTTAATGGTCTTAGCCGCCAGATTGTGAGATATCAGTCATCGAT
GAACGATGCAACAAACACTATGCTTCAACAGATACAGCAGATGAGTAATGCACCTAGCCA
TGAATCTCTCTCAAGCAATAATGGTAGCTTTCTTTTGGGCGATGTTCCAAATTCTAACAT
TTCAGACAATGGGAGCTCCTCAAATGGATCACCTGAAGTTACATTGGCAGATGTTTCATC
CATTCCTGCAGGCTTCTATCCTGCAATGAAGTACCACGAGCCTTGTGAAACAAATCAAGT
CATGGAGACCAATTTGCCTTTTTCTCAAGGTGATCTCTTACCTCCAACGCAAGGAGCAGC
AGCTTCTGGGAGCTCAAGTTCGGATCTTGTTGGATGCGAGACAGATAATGGAGAGTGTTT
GGATCCAATAATGGCTGTTTTGGATGGCGCACTCGAACTAGAAGCTGATACCTTAAATGA
GTTGCTTCCTGA

Last Updated on 06.12.2018 16:11