Genotyping and primer details


Genotyping and primer details for SALK_096866 - At1g35680
Line ID SALK_096866
Locus At1g35680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:13209064 of line SALK_096866 Chr1:13209064
   
Insertion allele amplicon R204 - CG27
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CG27: 5'-CAAATGTGCATATACCTGGTCATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:13209497
   
Insertion allele amplicon LBb3 - CG28
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CG28: 5'-GATAACACTGCGGATTTATTCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:13208705
   
wt amplicon (over InsPos) CG28 - CG27
wt amplicon start Chr1:13208705
wt amplicon end Chr1:13209497
wt amplicon size 793 bp
Locus specific primer CG27: 5'-CAAATGTGCATATACCTGGTCATC-3'
2nd locus specific primer CG28: 5'-GATAACACTGCGGATTTATTCTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11