Genotyping and primer details

Genotyping and primer details for SALK_098698c - At1g22130
Line ID SALK_098698c
Locus At1g22130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7813478 of line SALK_098698c Chr1:7813478
Insertion allele amplicon LBb3 - 01el
Locus specific primer 01el: 5'-AAGAACGAGAGAGGTACGCTAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7813934
wt amplicon (over InsPos) 02ev - 01el
wt amplicon start Chr1:7813285
wt amplicon end Chr1:7813934
wt amplicon size 650 bp
Locus specific primer 01el: 5'-AAGAACGAGAGAGGTACGCTAAAA-3'
2nd locus specific primer 02ev: 5'-TCAAGTTCCTAGATTTCCGATTTC-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11