Sequences from confirmation amplicon


Confirmation sequences for line SALK_098698c and hit At1g22130 are shown.
Line ID SALK_098698c
Locus At1g22130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7813478
Primers used for PCR 01el - LBb3, show primer details
   
Sequence Name 41-K110394-SALK_098698C-01el
GenBank Accession LN485309 [GenBank]
Sequencing primer gene specific primer, 01el: 5'-AAGAACGAGAGAGGTACGCTAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K110394-SALK_098698C-01el
TTTATAATTACTACATTTTTAGTAAATTTTGACTTTGGTTATTTGATTTGTTTTCTTTCA
GTGCTCTATACTTTCCTGACCAGAATAGACGCCCGTATGTATATCAGTTTTTCTTTTGTT
ACATTACACTTGATCATATGGGTATAATAAATTTTTCATGACCCTCTCTTTTCTTTTTTG
GTTTGTTTGCAGAGATATTCAAAACAAAGAGGTTAGTTCTAATCATAGTGATTAGTTTGT
TCCTCATTATGTTGTTTCCTTAAAGTCAAGAATAATGTTGTTGTTATGTGAAAGTGTCTA
CTAAGGATTCTGCAGCAACTCAAGACTGAAAATGACATCGCTCTTCAAGTCACGAAGTCC
GTAAATTCCTTCGGTTTGTCGTTATAATTGATTTTATAATTTTCATATGTTTAGACAAAA
TTGTGGAGCAACACATTACAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 33-K110393-SALK_098698C-R210
GenBank Accession LN485184 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K110393-SALK_098698C-R210
CGTCATTTGTTTACCCCAATATATCCTGTAATGTGTTGCTCCACAATTTTGTCTAAACAT
ATGAAAATTATAAAATCAATTATAACGACAAACCGAAGGAATTTACGGACTTCGTGACTT
GAAGAGCGATGTCATTTTCAGTCTTGAGTTGCTGCAGAATCCTTAGTAGACACTTTCACA
TAACAACAACATTATTCTTGACTTTAAGGAAACAACATAATGAGGAACAAACTAATCACT
ATGATTAGAACTAACCTCTTTGTTTTGAATATCTCTGCAAACAAACCAAAAAAGAAAAGA
GAGGGTCATGAAAAATTTATTATACCCATATGATCAAGTGTAATGTAACAAAAGAAAAAC
TGATATACATACGGGCGTCTATTCTGGTCAGGAAAGTATAGAGCACTGAAAGAAAACAAA
TCAAATAACCAAAGTCAAAATTTACTAAAAATGTAGTAATTTATAAAACTTGGAAAAGAA
AGTAGTTTTTAGCGTACCTCTCTCGTTC

Last Updated on 06.12.2018 16:11