Genotyping and primer details


Genotyping and primer details for SALK_098982c - At5g09880
Line ID SALK_098982c
Locus At5g09880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3085075 of line SALK_098982c Chr5:3085075
   
Insertion allele amplicon LBb3 - 41dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 41dr: 5'-CTTTGATCTCTCTCTGGATCTGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3084561
   
wt amplicon (over InsPos) 07ep - 41dr
wt amplicon start Chr5:3084561
wt amplicon end Chr5:3085291
wt amplicon size 731 bp
Locus specific primer 41dr: 5'-CTTTGATCTCTCTCTGGATCTGCT-3'
2nd locus specific primer 07ep: 5'-CTAGATATCGATCAAACAGGCACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11