Genotyping and primer details


Genotyping and primer details for SALK_104995c - At5g61760
Line ID SALK_104995c
Locus At5g61760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24814419 of line SALK_104995c Chr5:24814419
   
Insertion allele amplicon LBb3 - 17ck
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17ck: 5'-ATTTTTAGATTTTGCGTTTGGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24815007
   
wt amplicon (over InsPos) 25dn - 17ck
wt amplicon start Chr5:24813843
wt amplicon end Chr5:24815007
wt amplicon size 1165 bp
Locus specific primer 17ck: 5'-ATTTTTAGATTTTGCGTTTGGTTG-3'
2nd locus specific primer 25dn: 5'-CAAAACCCAACTAGTCCAAGAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11