Genotyping and primer details


Genotyping and primer details for SALK_105023c - At5g04160
Line ID SALK_105023c
Locus At5g04160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1143909 of line SALK_105023c Chr5:1143909
   
Insertion allele amplicon LBb3 - 18ke
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18ke: 5'-GCCTTTTTGTTCTGTTCTCAATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1144323
   
wt amplicon (over InsPos) 13ll - 18ke
wt amplicon start Chr5:1143365
wt amplicon end Chr5:1144323
wt amplicon size 959 bp
Locus specific primer 18ke: 5'-GCCTTTTTGTTCTGTTCTCAATTC-3'
2nd locus specific primer 13ll: 5'-ATGTACTGATGTTGTTTTGCAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11