Sequences from confirmation amplicon


Confirmation sequences for line SALK_105023c and hit At5g04160 are shown.
Line ID SALK_105023c
Locus At5g04160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1143909
Primers used for PCR 18ke - LBb3, show primer details
   
Sequence Name 61-K113814-SALK_105023C-R210
GenBank Accession LN485766 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K113814-SALK_105023C-R210
CATAACTATCAATAAGATCGTCTCTTCACAATGATACAAATTCAAAACATACAAAACAAG
GATATGATATAACCACTTGACAAGCTTCAGAGAAGTACAAAAACAGTATAAAGATATCAC
CTTTACGGAGAAATAAGGAAGGTTAGATCCCAAAATGATGTTCTACCAAATTCTACACCT
GATTAAACCATAAAAGGCTGAAGCTTTTTAGATAACAACAACACTCAAATTTGCCCTGAT
TTCAAAGTTTAGACCTTTGTCCAAGTTTAGGTCTTTCAACATTAACATCTATTTACGGAT
CAGTGATCTGAATCCAAATTGGATCTTTCCAAAAAAAAACATGAATTTCAAATCTAGGGA
GGTCCAGGAAACCTAACATTCAACACTGACATTGTTAAGAGAATTGAGAACAGAACAAAA
AG
   
Sequence Name 74-K113815-SALK_105023C-18ke
GenBank Accession LN486106 [GenBank]
Sequencing primer gene specific primer, 18ke: 5'-GCCTTTTTGTTCTGTTCTCAATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K113815-SALK_105023C-18ke
AGTTTCTGGACCTCCCTAGATTTGAATTCATGTTTTTTTTTGGAAAGATCCAATTTGGAT
TCAGATCACTGATCCGTAAATAGATGTTAATGTTGAAAGACCTAAACTTGGACAAAGGTC
TAAACTTTGAAATCAGGGCAAATTTGAGTGTTGTTGTTATCTAAAAAGCTTCAGCCTTTT
ATGGTTTAATCAGGTGTAGAATTTGGTAGAACATCATTTTGGGATCTAACCTTCCTTATT
TCTCCGTAAAGGTGATATCTTTATACTGTTTTTGTACTTCTCTGAAGCTTGTCAAGTGGT
TATATCATATCCTTGTTTTGTGTGTTTTGAATTTGTATCATTGTGAAGAGACGATCTTAT
TGATAGTTTATTGCTATATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAA
ATCCTGTTTGATGGTGGTTCCGAAATCGGCAAA

Last Updated on 06.12.2018 16:11