Genotyping and primer details

Genotyping and primer details for SALK_107662c - At1g17290
Line ID SALK_107662c
Locus At1g17290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5924761 of line SALK_107662c Chr1:5924761
Insertion allele amplicon LBb3 - 28ty
Locus specific primer 28ty: 5'-CAGAGATGTTGGAACAAAGATTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5925377
wt amplicon (over InsPos) 18uu - 28ty
wt amplicon start Chr1:5924455
wt amplicon end Chr1:5925377
wt amplicon size 923 bp
Locus specific primer 28ty: 5'-CAGAGATGTTGGAACAAAGATTCA-3'
2nd locus specific primer 18uu: 5'-GAACACCTTGTTTTGAAGTTGACA-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11