Genotyping and primer details


Genotyping and primer details for SALK_108832 - At1g51710
Line ID SALK_108832
Locus At1g51710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19176384 of line SALK_108832 Chr1:19176384
   
Insertion allele amplicon LBb3 - 03fc
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 03fc: 5'-AAAATGACTGATGCAGAGGTACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19176560
   
wt amplicon (over InsPos) 01fo - 03fc
wt amplicon start Chr1:19175460
wt amplicon end Chr1:19176560
wt amplicon size 1101 bp
Locus specific primer 03fc: 5'-AAAATGACTGATGCAGAGGTACAA-3'
2nd locus specific primer 01fo: 5'-CAACTCTCATCCCATAACTCCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11