Genotyping and primer details

Genotyping and primer details for SALK_111817c - At5g65600
Line ID SALK_111817c
Locus At5g65600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26217730 of line SALK_111817c Chr5:26217730
Insertion allele amplicon LBb3 - 03oz
Locus specific primer 03oz: 5'-AGTTTCCTTCATAAACTGCTCCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26217049
wt amplicon (over InsPos) 04rd - 03oz
wt amplicon start Chr5:26217049
wt amplicon end Chr5:26217938
wt amplicon size 890 bp
Locus specific primer 03oz: 5'-AGTTTCCTTCATAAACTGCTCCAA-3'
2nd locus specific primer 04rd: 5'-AACATCTCAGGTTGGTTGGATTAC-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11