Genotyping and primer details


Genotyping and primer details for SALK_112170 - At2g27210
Line ID SALK_112170
Locus At2g27210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11634069 of line SALK_112170 Chr2:11634069
   
Insertion allele amplicon LBb3 - 18vt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18vt: 5'-TGTCTTAATATGTGGGGTGTCGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11633465
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11