Genotyping and primer details


Genotyping and primer details for SALK_112206 - At5g62540
Line ID SALK_112206
Locus At5g62540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25105379 of line SALK_112206 Chr5:25105379
   
Insertion allele amplicon LBb3 - 58bk
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 58bk: 5'-AATCAGTCTGTTGTGTTCATCAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25104979
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11