Sequences from confirmation amplicon


Confirmation sequences for line SALK_112206 and hit At5g62540 are shown.
Line ID SALK_112206
Locus At5g62540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25105379
Primers used for PCR 58bk - LBb3, show primer details
   
Sequence Name 17-K108069-SALK_112206C-58bk
GenBank Accession LN484736 [GenBank]
Sequencing primer gene specific primer, 58bk: 5'-AATCAGTCTGTTGTGTTCATCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K108069-SALK_112206C-58bk
GAGGATATCCGAACAACCCCCGATTGTTCGGTTTGTTTCACGGATGTTTCATCCAAACAG
TAAGCCCAAGCATTCAGTGAACAACATAGTAATAGACCAATTAGGAAATTCGACTTTGAT
AATTGAACTTTGGTGCAGTTTATGCTGATGGAAGCATTTGCTTGGATATCTTGCAAAACC
AGTGGAGCCCCATCTACGACGTTGCTGCAGTTCTCACATCAATTCAGGTTTTGACTTCTG
AATTCTTCATTTGTATCTTTTGCATTGTGGAGGTTATGCATGAACTAAACCAAGACCTTT
TGTTTTACAGTCTTTGCTCTGTGACCCAAACCCTGACTCACCAGCGATATATTGTGGTGT
AAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGG
TGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCT
GAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGA
TGGTGGTTCCGAAACANGCAAAA
   
Sequence Name 28-K108070-SALK_112206C-R210
GenBank Accession LN485077 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K108070-SALK_112206C-R210
CGTCATTTGTTACACCCAATATATCGCTGGTGAGTCAGGGTTTGGGTCACAGAGCAAAGA
CTGTAAAACAAAAGGTCTTGGTTTAGTTCATGCATAACCTCCACAATGCAAAAGATACAA
ATGAAGAATTCAGAAGTCAAAACCTGAATTGATGTGAGAACTGCAGCAACGTCGTAGATG
GGGCTCCACTGGTTTTGCAAGATATCCAAGCAAATGCTTCCATCAGCATAAACTGCACCA
AAGTTCAATTATCAAAGTCGAATTTCCTAATTGGTCTATTACTATGTTGTTCACTGAATG
CTTGGGCTTACTGTTTGGATGAAACATCCGTGAAACAAACCGAACAATCGGGGGTTTGTT
CGGATAATCCTCGGTGAAGTGAAGAGTCAACTTGAAAGTACCTGATGAACACAACGNACT
GAT

Last Updated on 06.12.2018 16:11