Genotyping and primer details


Genotyping and primer details for SALK_113426c - At1g55890
Line ID SALK_113426c
Locus At1g55890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20901874 of line SALK_113426c Chr1:20901874
   
Insertion allele amplicon LBb3 - 63cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 63cs: 5'-CACAATCTCCTCAGCTTCTTCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20902497
   
wt amplicon (over InsPos) 29dw - 63cs
wt amplicon start Chr1:20901539
wt amplicon end Chr1:20902497
wt amplicon size 959 bp
Locus specific primer 63cs: 5'-CACAATCTCCTCAGCTTCTTCTCT-3'
2nd locus specific primer 29dw: 5'-AGAAATTCAAGAAGGCATGTGAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11