Sequences from confirmation amplicon


Confirmation sequences for line SALK_113426c and hit At1g55890 are shown.
Line ID SALK_113426c
Locus At1g55890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20901874
Primers used for PCR 63cs - LBb3, show primer details
   
Sequence Name 91-K109033-SALK_113426C-63cs
GenBank Accession LN486341 [GenBank]
Sequencing primer gene specific primer, 63cs: 5'-CACAATCTCCTCAGCTTCTTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K109033-SALK_113426C-63cs
CACTAACTGCTGCACGTTGTTTGACCGACAGATACCGCTTACTGAATGTCTCCCTGTAGA
GCTCAATCGCAGACTCAAAGTCACCTGCTTTACACATAGCAGGAAGCAATAAAGCAAAGG
TAGCTTTATCAGGACGATAACCATGCTTCACAATTTCTTTGTACCACGCTTCAGCTTCGT
CCATTTTTCCTTCGTTGATAGATCCTCTAATCATAGCATTGAAACTGAAAACATCAGGTT
TGAGCCCACTAGCCTTAAGTTCTCCAAAGAGATTTACTAGCTCTTTCGATTTCGCCTCAT
TGGCTAATCCAAGCAGCCTAGCATTGTAAGTCCTGATGTCTATTGCTACATTCTTCTCCA
CCATTTTAGCCCATATCTCTTCCCCTAGCTCAAACTGTCCCTTTAGATACGAAGACAGTA
AAAGTGTGTTGAATGTAACTATATCAGGTTTTAGTCCCTTGTTCTCAATCTCATCAAGCA
ATGCAACAGCTTCAGGTAAAGAATCCTTCTCACACAATGCCTTAATCAAAGTATTGTAAG
ATACAATATCTGGTTTGATTGATAACTTTCCAGGATATATTGTGGTGTAAACAAATTGAC
GCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTT
TCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCA
GCAAGCGGTCCACGCTGGTTTGCCCCAGCAAGCGAAAATCCTGTTTGATG
   
Sequence Name 75-K109032-SALK_113426C-R210
GenBank Accession LN486125 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K109032-SALK_113426C-R210
GTCATTTGTTACCCACAATATATCCTGGAAAGTTATCAATCAAACCAGATATTGTATCTT
ACAATACTTTGATTAAGGCATTGTGTGAGAAGGATTCTTTACCTGAAGCTGTTGCATTGC
TTGATGAGATTGAGAACAAGGGACTAAAACCTGATATAGTTACATTCAACACACTTTTAC
TGTCTTCGTATCTAAAGGGACAGTTTGAGCTAGGGGAAGAGATATGGGCTAAAATGGTGG
AGAAGAATGTAGCAATAGACATCAGGACTTACAATGCTAGGCTGCTTGGATTAGCCAATG
AGGCGAAATCGAAAGAGCTAGTAAATCTCTTTGGAGAACTTAAGGCTAGTGGGCTCAAAC
CTGATGTTTTCAGTTTCAATGCTATGATTAGAGGATCTATCAACGAAGGAAAAATGGACG
AAGCTGAAGCGTGGTACAAAGAAATTGTGAAGCATGGTTATCGTCCTGATAAAGCTACCT
TTGCTTTATTGCTTCCTGCTATGTGTAAAGCAGGTGACTTTGAGTCTGCGATTGAGCTCT
TCAAGGAGACATTCAGTAAGCGGTATCTTGTCGGTCAAACAACGTTGCAGCAGTTAGTTG
ATGAATTGGTGAAAGGGTCTAAGAGAGAAGAAGCTGAGGAGATTG

Last Updated on 06.12.2018 16:11