Genotyping and primer details

Genotyping and primer details for SALK_115539 - At3g62590
Line ID SALK_115539
Locus At3g62590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:23148404 of line SALK_115539 Chr3:23148404
Insertion allele amplicon LBb3 - 59bk
Locus specific primer 59bk: 5'-GCTAACATCTTTTCATTTGGAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:23147667
wt amplicon (over InsPos) 04da - 59bk
wt amplicon start Chr3:23147667
wt amplicon end Chr3:23148512
wt amplicon size 846 bp
Locus specific primer 59bk: 5'-GCTAACATCTTTTCATTTGGAAGC-3'
2nd locus specific primer 04da: 5'-TTGGCTTACTCTGTTTGGCTTATT-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11