Sequences from confirmation amplicon


Confirmation sequences for line SALK_115539 and hit At3g62590 are shown.
Line ID SALK_115539
Locus At3g62590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23148404
Primers used for PCR 59bk - LBb3, show primer details
   
Sequence Name 21-K108069-SALK_115539C-59bk
GenBank Accession LN484875 [GenBank]
Sequencing primer gene specific primer, 59bk: 5'-GCTAACATCTTTTCATTTGGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K108069-SALK_115539C-59bk
GAGTTGATCTCTAGTTTCCTCCTCAATACTCATCTTGTCACAAAGGTCTGACCTTTTACG
CCCCCTTAATGAATAAATCTCTTGCATTTCCTCGCCAATCCCTTTGTAAGATTGTGTCAG
ACTCCAAACATATTTCAGTGAAATCATGAAAGAAATTGTTACCAAGCACTCTTGTACGAA
GATACTTCCTGTCTTTCTCTAAAAATAATCCATGTTTGATAGATTCTATTACAGAATAGG
CCTAAGCTGCTTCACTTGAAGAAACTAAAATGTGCTTGTGAATGGTGAAAGACCACACTG
TAGACACTCATTATTAACAATCTAATTGGGAGAAAGAAGATGATCACTAGTTGACTACTC
CTTGATGCCACGAATCTTGTTATGAGTTTCAAAGAATCTCTCCCACTAAATAAAATGTTT
ATAATAAAGAACTTGCGCAGCCATTGATCCCGCTCAGCTTTCATCTGCTTCAGCTCCTTC
CGGATCACATGCCTCAAGGCCTTCAGGTAAGAGCTCATGTCATGGTTTCTTTTGATTTTT
CCTTCCGACCCGTAAGAACGACGATCACTGAGAATCTCTAGGGGGTGTGGTGAGTTAAAG
AAGAGAATCTTTGCAGCCCTTAGACTTTTCTCTGTTTCATCGGTATTCTTAGATGCTAAG
AGATAGAGACCACTTCCGGGAGGGTTGTAAACAAGCGATGGTTGTACTGGGGTGTAAACA
AAAACGACAAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACA
TTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAG
CTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG
CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 32-K108070-SALK_115539C-R210
GenBank Accession LN485160 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K108070-SALK_115539C-R210
GCGTATTTGTTACCCACAATATATCCTTGTCGTTTTTGTTTACACCCCAGTACAACCATC
GCTTGTTTACAACCCTCCCGGAAGTGGTCTCTATCTCTTAGCATCTAAGAATACCGATGA
AACAGAGAAAAGTCTAAGGGCTGCAAAGATTCTCTTCTTTAACTCACCACACCCCCTAGA
GATTCTCAGTGATCGTCGTTCTTACGGGTCGGAAGGAAAAATCAAAAGAAACCATGACAT
GAGCTCTTACCTGAAGGCCTTGAGGCATGTGATCCGGAAGGAGCTGAAGCAGATGAAAGC
TGAGCGGGATCAATGGCTGCGCAAGTTCTTTATTATAAACATTTTATTTAGTGGGAGAGA
TTCTTTGAAACTCATAACAAGATTCGTGGCATCAAGGAGTAGTCAACTAGTGATCATCTT
CTTTCTCCCAATTAGATTGTTAATAATGAGTGTCTACAGTGTGGTCTTTCACCATTCACA
AGCACATTTTAGTTTCTTCAAGTGAAGCAGCTTAGGCCTATTCTGTAATAGAATCTATCA
AACATGGATTATTTTTAGAGAAAGACAGGAAGTATCTTCGTACAAGAGTGCTTGGTAACA
ATTTCTTTCATGATTTCACTGAAATATGTTTGGAGTCTGACACAATCTTACAAAGGGATT
GGCGAGGAAATGCAAGAGATTTATTCATTAAGGGGGCGTAAAAGGTCAGACCTTTGTGAC
AAGATGAGTATTGAGGAGGAAACTAGAGATTCAACTTCCCAGCTAGATACCAAGAATGCA
TAGCAAGGCTTCCAAATGAAAGAAATGTTA

Last Updated on 06.12.2018 16:11