Genotyping and primer details

Genotyping and primer details for SALK_115549c - At2g43460
Line ID SALK_115549c
Locus At2g43460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18047095 of line SALK_115549c Chr2:18047095
Insertion allele amplicon LBb3 - 05jd
Locus specific primer 05jd: 5'-CTGATCATCAATTGTTTTGTTGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18047833
wt amplicon (over InsPos) 17km - 05jd
wt amplicon start Chr2:18046886
wt amplicon end Chr2:18047833
wt amplicon size 948 bp
Locus specific primer 05jd: 5'-CTGATCATCAATTGTTTTGTTGCT-3'
2nd locus specific primer 17km: 5'-CTGTCAGAAGGAAGTCCTTGATCT-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11