Genotyping and primer details


Genotyping and primer details for SALK_116659 - At5g50720
Line ID SALK_116659
Locus At5g50720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20633781 of line SALK_116659 Chr5:20633781
   
Insertion allele amplicon LBb3 - 47fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 47fx: 5'-TGTTAAAACGTTTCTGGATGACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20632890
   
wt amplicon (over InsPos) 27lt - 47fx
wt amplicon start Chr5:20632890
wt amplicon end Chr5:20634353
wt amplicon size 1464 bp
Locus specific primer 47fx: 5'-TGTTAAAACGTTTCTGGATGACTG-3'
2nd locus specific primer 27lt: 5'-ATCCTGGATTTTCATTTCATCATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11