Genotyping and primer details

Genotyping and primer details for SALK_117769 - At4g27890
Line ID SALK_117769
Locus At4g27890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13887093 of line SALK_117769 Chr4:13887093
Insertion allele amplicon LBb3 - 15rr
Locus specific primer 15rr: 5'-TTCTTGTGTCTGGAAACTGGTATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13886422
wt amplicon (over InsPos) 06tc - 15rr
wt amplicon start Chr4:13886422
wt amplicon end Chr4:13887581
wt amplicon size 1160 bp
Locus specific primer 15rr: 5'-TTCTTGTGTCTGGAAACTGGTATG-3'
2nd locus specific primer 06tc: 5'-AAAAACGCAATTTCACGATTTTAC-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11