Genotyping and primer details


Genotyping and primer details for SALK_121082c - At5g65640
Line ID SALK_121082c
Locus At5g65640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26237712 of line SALK_121082c Chr5:26237712
   
Insertion allele amplicon LBb3 - 57wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 57wm: 5'-AAGAATGGAACTGTCGACTCAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26237133
   
wt amplicon (over InsPos) 13xf - 57wm
wt amplicon start Chr5:26237133
wt amplicon end Chr5:26238193
wt amplicon size 1061 bp
Locus specific primer 57wm: 5'-AAGAATGGAACTGTCGACTCAAAT-3'
2nd locus specific primer 13xf: 5'-AAATGATTTATCACTTTTCAGCCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11